In this study, the dexamethasone suppression test (DST) and thyroid releasing hormone (TRH) test were applied to 29 patients with major depression (MD) and 24 healthy controls to assess their hypothalamic-pituitary-adrenal (HPA) and hypothalamic-pituitary-thyroid (HPT) axis functions. Predexamethasone 9.00 a.m. and 11.00 p.m., and post-dexamethasone 4.00 p.m. mean cortisol levels were significantly higher in MD patients, than the control group. All TSH responses to TRH and TSH values were significantly lower in MD patients. Sensitivity and specificity of TRH test were 79.3% and 100%, for DST these values were 44.8%, and 91.7% respectively. By the combination of both tests the sensitivity and specificity were %35.4 and %91.7. We found that predexamethasone at 4.00 p.m. mean cortisol levels; basal TSH and at 30 minute TSH response to TRH could distinguish the MD and healthy control groups. But we could not show any discriminative HPA and HPT axis finding for MD subgroups.